175 Axonal Loss in Narcolepsy Type 1 Figure 3. Axon counting results. (A) Exemplary midbrain section of a donor with narcolepsy type 1 with the reticular formation (RF) and pyramidal tract (P) ROIs. (B-C) Zoomed version of the ROIs in A. Each yellow dot represents one counted axon. Data on the average axon counts are displayed for each donor in the graph, and group results are expressed as mean ± standard error of the mean (SEM). (D) Exemplary section of a donor with narcolepsy type 1 with the proximal corpus callosum (P-CC) and anterior cingulate gyrus (ACC) ROIs. (E-F) Zoomed version of the ROIs in D. Each yellow dot represents one counted axon in the main fibre direction and a green dot in the perpendicular fibre direction. Data on the average axon counts for each donor are displayed in the graph, and group results are expressed as mean ± standard error of the mean (SEM). The dashed line represents the white-grey matter border. (G) Exemplary section of a donor with narcolepsy type 1 with the cerebellum ROI. (H) Zoomed version 6
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