165 Axonal Loss in Narcolepsy Type 1 Figure 1. Examples of all regions of interest in the SMI312 axonal integrity staining. (A) Midbrain section with the reticular formation (RF) and the pyramidal tract (P) ROIs. The substantia nigra and red nucleus served as anatomical landmarks. (B) Corpus callosum section with the proximal corpus callosum (P-CC), distal corpus callosum (D-CC), anterior cingulate gyrus (ACC) and cingulate sulcus (CS). The dashed line represents the constant three mm distance from the origin of the cingulate sulcus that was used to draw the CS ROI. (C) Occipital section where the band of Gennari served as the anatomical landmark to distinguish the primary visual cortex (V1) from the secondary visual cortex (V2). (D) The cerebellum section with the vermis (CB) where the dentate nucleus served as an anatomical landmark. The scale bar represents two mm in all sections. Tissue processing Tissue processing and staining procedures are described in Appendix A. In brief, all previously described tissue blocks were paraffin-embedded and cut at 20 µm, except for the midbrain which had already been cut at 6 µm for a 6
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