180 Chapter 8 Self-sampling at home Resuspension of self-collected sample DNA extraction Bisulphite conversion Transport of self-collected sample to screening laboratorium Quantitative methylation specific PCR (qMSP) 200 ng of extracted DNA Cytosine DNMTs 5-Methylcytosine Bisulphite conversion Fluoresence Cycles Positive Negative Threshold qMSP* ThinPrepTM PreservCytTM ThinPrepTM PreservCytTM ThinPrepTM PreservCytTM 10% of self-collected sample Double beads DNA isolation Figure 8.3 Workflow for methylation-based triage using self-collected samples and its steps that may be optimised. * qMSP with gene targets and a housekeeping gene Abbreviations: DNA, deoxynucleic acid; ng, nanogram; DNMTs, DNA methyltransferases; CH3, methylgroup; qMSP, quantitative methylation specific PCR
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