66 Chapter 3 SUPPLEMENTARY MATERIAL Introduction: 18F-flortaucipir R 1 The validation against the gold standard 15O-H 2O is currently lacking for 18F-flortaucipir R1. Notwithstanding, for other PET tracers such as 11C-PIB[1] and 18F-Florbetapir[2], R1 was significantly associated with 15O-H 2O estimates. A previous study has demonstrated that R1 obtained from a dynamic 18F-florbetapir scan is well correlated with the gold standard 15O-H 2O SUVr and therefore may serve as a measure for rCBF[2]. In a recent study from our group (Tuncel et al., in preparation), we directly compared R1 obtained from 18F-flortaucipir and 18F-florbetapir in the same subjects. We observed good correlation and ICC’s in all cortical regions. It was observed R1 obtained from a 18F-flortaucipir PET scan is similar to the R 1 obtained from a 18F-florbetapir PET scan. Henceforth suggesting that R1 obtained from a 18F-flortaucipir could be considered as a proxy of rCBF. Taken together, pending further validation, these results suggest that 18F-flortaucipir R 1 can be used to represent rCBF. Methods: Diagnostic procedures All subjects underwent a standardized dementia screening, including medical history, extensive neuropsychological assessment, physical and neurological examination, lumbar puncture (LP), blood tests, electroencephalography and brain magnetic resonance imaging (MRI)[3, 4]. Diagnosis was established by consensus in a multidisciplinary meeting and met criteria according to the National Institute on Aging and Alzheimer’s Association (NIA-AA)[3, 5]. The SCD diagnosis was established based on self-reported cognitive complaints, while all clinical and cognitive investigations were normal[6]. Methods: Exclusion criteria Exclusion criteria for all participants were (1) non-AD dementia diagnosis, (2) significant cerebrovascular disease as assessed by MRI, (3) major traumatic brain injury, (4) major psychiatric or neurological disorders (other than AD), (5) recent substance abuse. Methods: Image Acquisition and Analyses In short, the first 60 minute dynamic scan started simultaneously with a bolus injection of 238±12 MBq 18F-flortaucipir (injected mass 1.1±0.9 µg). The second PET scan was co-registered to the first dynamic PET scan using Vinci (Volume Imaging in Neurological Imaging) software[7] and PET list mode data were rebinned into a total of 29 frames (1x15, 3x5, 3x10, 4x60, 2x150, 2x300, 4x600 and 10x300 seconds).
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