95 DNA methylation testing for endometrial cancer detection in patient-friendly sample types reduce hands-on time, costs and the amount of DNA needed. Self-samples and urines were collected at home, which is an appropriate setting to evaluate the use of homebased sampling for endometrial cancer detection. The collection of paired sample types allowed a comprehensive comparison of their performance. Our study demonstrates that DNA methylation testing allows endometrial cancer detection with high sensitivity and specificity using a three-marker panel of methylated genes in patient-friendly sample types that can be collected at home. Following validation in additional cohorts, including individuals presenting with postmenopausal bleeding and asymptomatic women at-risk for endometrial cancer, methylation testing could be valuable as a preselection method to inexpensively determine who needs to undergo invasive endometrial tissue sampling and facilitate timely diagnosis. ACKNOWLEDGEMENTS The authors would like to thank the URIC biobank team. The authors also thank Danique Wajon for technical assistance. FUNDING This research was in part funded by the Hanarth Foundation and Weijerhorst Foundation. The funders had no role in the design of the study; in the collection, analysis or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. DATA AVAILABILITY STATEMENT The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request. CONFLICT OF INTEREST Renske D.M. Steenbergen and Daniëlle A.M. Heideman have a minority share in Selfscreen B.V., a spin-off company of Amsterdam UMC, location VUmc. Self-screen B.V. holds patents and products related to the work (methylation markers for cervical screening). The remaining authors declare no potential conflicts of interest. AUTHORS’ CONTRIBUTIONS BMMW: Resources, data curation, formal analysis, investigation, visualization, methodology, writing-original draft. RvdH: Conceptualization, resources, data curation, formal analysis, investigation, visualization, methodology, writing–original draft. APvS: Investigation, methodology, writing-review and editing. MDJMvG: Resources, writingreview and editing. JCK: Resources, writing-review and editing. JWT: Resources, writing4
RkJQdWJsaXNoZXIy MjY0ODMw