86 Chapter 4 Urine Scrape Self-sample ADCYAP1 GHSR ZIC1 SST HAND2 GALR1 CDO1 CDH13 BHLHE22 ● ● **** −15 −10 −5 0 5 10 Control EC ● **** −15 −10 −5 0 5 10 Control EC ● ● **** −15 −10 −5 0 5 10 Control EC ● ● ● ● ● **** −15 −10 −5 0 5 10 Control EC ● ● ● **** −15 −10 −5 0 5 10 Control EC ● ● **** −15 −10 −5 0 5 10 Control EC ● ● ● **** −15 −10 −5 0 5 10 Control EC ● ● **** −15 −10 −5 0 5 10 Control EC ● ● ● ● ● ● **** −15 −10 −5 0 5 10 Control EC ● ● **** −15 −10 −5 0 5 10 Control EC ● ● **** −15 −10 −5 0 5 10 Control EC ● ● ● ● **** −15 −10 −5 0 5 10 Control EC ● ● ● **** −15 −10 −5 0 5 10 Control EC ● ● ● ● **** −15 −10 −5 0 5 10 Control EC ● ● ● ● ** −15 −10 −5 0 5 10 Control EC ● ● ● **** −15 −10 −5 0 5 10 Control EC ● ● ● **** −15 −10 −5 0 5 10 Control EC ● ● ● ● **** −15 −10 −5 0 5 10 Control EC ● ● ● ● **** −15 −10 −5 0 5 10 Control EC ● ● ● ● *** −15 −10 −5 0 5 10 Control EC ● ● ● ● ● ** −15 −10 −5 0 5 10 Control EC ● ● ● ● ● ● ** −15 −10 −5 0 5 10 Control EC ● ● ● ● ** −15 −10 −5 0 5 10 Control EC ● ● ● **** −15 −10 −5 0 5 10 Control EC **** −15 −10 −5 0 5 10 Control EC ** −15 −10 −5 0 5 10 Control EC ● ● ● ● ● **** −15 −10 −5 0 5 10 Control EC Methylation level (log2 Ct ratio) Control (urine n=100; self-sample n=107; scrape n=110) EC (n=103) Figure 1: DNA methylation levels of ADCYAP1, BHLHE22, CDH13, CDO1, GALR1, GHSR, HAND2, SST, and ZIC1 in urine, cervicovaginal self-samples and clinician-taken cervical scrapes from healthy female controls (n = 100; n = 107; n = 110, respectively) and endometrial cancer patients (n = 103). DNA methylation levels are shown by the 2log-transformed Ct ratios. Boxplots illustrate medians with lower and upper quartile and range whiskers. Outliers are indicated with black circles. A P-value of < .05 was considered statistically significant. **: p ≤ 0.01; ***: p ≤ 0.001; ****: p ≤ 0.0001. Ct, cycle threshold; EC, endometrial cancer.
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