183 Dynamics of methylated cell-free DNA in the urine of non-small cell lung cancer patients Day 1 morning afternoon evening morning afternoon evening 1 10 100 cfDNA concentration (ng DNA / mL urine) Day 2 Figure 1: Logarithmic representation of cfDNA concentrations (ng/mL urine) measured at different collection time points, illustrating the median and IQR of each collection time point. Outliers are indicated by bold circles located outside the whiskers of the boxplot. No significant differences were found within or between the days. cfDNA = cell-free DNA; IQR = interquartile range. Sample time point cfDNA concentration (ng DNA/mL urine) 5 10 0 20 40 60 140 180 20 60 100 5 10 15 20 0 200 400 0 100 200 50 100 50 100 150 2 6 10 0 1 2 0 4 8 0 1 2 3 50 100 150 0 40 80 5 15 4 5 6 5 15 25 20 40 5 15 25 0 50 100 20 60 10 30 day 1 day 2 Sex Female Male m a e m a e Figure 2: Conditional scatterplots displaying the between- and within-subject variability of the urinary cfDNA concentration of each NSCLC patient across the six sampled time points (m = morning, a = afternoon, e = evening), stratified by sex (pink square = female, blue circle = male). cfDNA = cell-free DNA; NSCLC = non-small cell lung cancer. 7
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