144 Chapter 5 Supplemental Table 2: Analytical validation of multiplex quantitative methylation-specific PCR assays. Multiplex Target Slope R2 Efficiency (%) 1 GHSR -3,38 1,00 97,52 1 SST -3,24 0,99 103,69 1 ZIC1 -3,23 0,99 104,00 1 ACTB -3,39 0,99 97,26 2 CDO1 -3,21 0,99 104,78 2 MAL -3,28 0,98 101,89 2 PRDM14 -3,37 0,99 98,15 2 ACTB -3,38 0,99 102,39 3 C2CD4D -3,46 0,98 94,56 3 GALR1 -3,27 0,99 102,03 3 NRN1 -3,36 0,99 98,64 3 ACTB -3,38 0,99 97,76 Data is based on serial dilution series of bisulfite treated methylated DNA from the SiHa cell line (100, 50, 10, 5, 1, 0.5%) within the range of 20 to 0.1 ng.
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