143 Molecular analysis for ovarian cancer detection in patient-friendly samples Supplemental Table 1: Primer and probe information of multiplex quantitative methylation-specific PCR assays. Multiplex Target Forward primer Reverse primer Probe 5’Dye- 3’Quencher Amplicon size Amplicon location Tm (°C) 1 GHSR available upon request available upon request available upon request FAM - BHQ1 88 available upon request 63 1 SST GCGTTGGTTG CGTTGTTTATC CTACAAAAACTAACGAAATCTAAAATCCG CCGATAACACAACCCAAAA VIC - MGB-Eclipse® 84 Chr3: 187670184187670267 63 1 ZIC1 available upon request available upon request available upon request DFO - BHQ2 84 available upon request 63 2 CDO1 CGTTTTTTTTCGTTTTATTTTCGTCG CCTCCGACCCTTTTTATCTACG TGTGGTTCGCGACGTTGGGACGT FAM - BHQ1 69 Chr5: 115816927115816995 60 2 MAL CGCGTAGTATTAAGTAGAGAGGTTCG ACCGCCGACCCCTTCC CCACTAAACCGACGCTAATTCGACGCT DFO - BHQ2 85 Chr2: 9502511395025197 60 2 PRDM14 TTACGTGTTATTGTCGGGGATTC ATATCTATTCCTAATACCTAAAAACGAAACG AAACGCCTTAAACGCTAAAAAACTTCGCCTC JOE - BHQ2 88 Chr8: 7007171870071805 60 3 C2CD4D CGTGGGTCGTAGTTGGTAGTATAG AACCCGCACTCGCCG CGCCGAACCGCCC DFO - MGB-Eclipse® 84 Chr1: 151838369151838452 59 3 GALR1 available upon request available upon request available upon request FAM - BHQ1 65 available upon request 59 3 NRN1 available upon request available upon request available upon request JOE - BHQ1 65 available upon request 59 1,2 ACTB available upon request available upon request available upon request CY5 - MGB-Eclipse® 68 available upon request 63, 60 3 ACTB available upon request available upon request available upon request CY5 - MGB-Eclipse® 108 available upon request 59 A {base} indicates a locked nuclear acid (LNA). Amplicon locations are based on GRCh38.109. Sequences not provided are available upon reasonable request from Self-screen B.V. 5
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