132 Chapter 5 SUPPLEMENTAL FIGURES Table of contents Supplemental Figure 1 DNA methylation levels of C2CD4D, CDO1, GALR1, GHSR, MAL, NRN1, PRDM14, SST, and ZIC1 in high-grade serous ovarian cancer (n=35) and normal fallopian tube tissue (n=22). DNA methylation levels are shown by 2log-transformed Cq ratios. Violin plots represent medians with lower and upper quartile and range whiskers. A p-value of <0.05 was considered statistically significant. ****: p < 0.0001. Cq = quantification cycle; HGSOC = high-grade serous ovarian cancer. Supplemental Figure 2 DNA methylation levels of C2CD4D, CDO1, GALR1, GHSR, MAL, NRN1, PRDM14, SST, and ZIC1 in full void (i.e. unfractionated) urine of healthy controls (n=30), and women diagnosed with a benign (n=27) or high-stage malignant ovarian mass (n=28). DNA methylation levels are shown by 2log-transformed Cq ratios. Violin plots represent medians with lower and upper quartile and range whiskers. A p-value of <0.05 was considered statistically significant. Cq = quantification cycle. Supplemental Figure 3 DNA methylation levels of C2CD4D, CDO1, GALR1, GHSR, MAL, NRN1, PRDM14, SST, and ZIC1 in urine supernatant of healthy controls (n=29), and women diagnosed with a benign (n=27) or high-stage malignant ovarian mass (n=29). DNA methylation levels are shown by 2log-transformed Cq ratios. Violin plots represent medians with lower and upper quartile and range whiskers. A p-value of <0.05 was considered statistically significant. Cq = quantification cycle. Supplemental Figure 4 DNA methylation levels of C2CD4D, CDO1, GALR1, GHSR, MAL, NRN1, PRDM14, SST, and ZIC1 in urine sediment of healthy controls (n=30), and women diagnosed with a benign (n=27) or high-stage malignant ovarian mass (n=29). DNA methylation levels are shown by 2log-transformed Cq ratios. Violin plots represent medians with lower and upper quartile and range whiskers. A p-value of <0.05 was considered statistically significant. Cq = quantification cycle. Supplemental Figure 5 DNA methylation levels of C2CD4D, CDO1, GALR1, GHSR, MAL, NRN1, PRDM14, SST, and ZIC1 in clinician-taken cervical scrapes of healthy controls (n=40), and women diagnosed with a benign (n=23) or high-stage malignant ovarian mass (n=24). DNA methylation levels are shown by 2log-transformed Cq ratios. Violin plots represent medians with lower and upper quartile and range whiskers. A p-value of <0.05 was considered statistically significant. Cq = quantification cycle. Supplemental Figure 6 DNA methylation levels of C2CD4D, CDO1, GALR1, GHSR, MAL, NRN1, PRDM14, SST, and ZIC1 in self-collected cervicovaginal samples of healthy controls (n=40), and women diagnosed with a benign (n=25) or high-stage malignant ovarian mass (n=28). Violin plots represent medians with lower and upper quartile and range whiskers. DNA methylation levels are shown by 2log-transformed Cq ratios. A p-value of <0.05 was considered statistically significant. Cq = quantification cycle. Supplemental Figure 7 The Spearman’s rank correlation coefficients (r) of methylation markers C2CD4D, CDO1, GHSR, and MAL between paired samples of 23 women diagnosed with ovarian cancer. The Spearman’s rank correlation coefficient was calculated based on 2logtransformed Cq ratios. Circle color and size indicate the degree of correlation (i.e. the larger and darker the circle, the more correlation).
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