107 DNA methylation testing for endometrial cancer detection in patient-friendly sample types Supplementary Table 3: Diagnostic performance of the nine individual methylation markers (univariable logistic regression) and optimal three-marker panels (multivariable logistic regression) for early-stage endometrial cancer detection (n=72) in the different sample types. Urine ADCYAP1 BHLHE22 CDH13 CDO1 GALR1 GHSR HAND2 SST ZIC1 CDH13+GHSR+SST AUC (non-CV; 95%-CI) 0.86 (0.80-0.92) 0.87 (0.81-0.93) 0.90 (0.85-0.95) 0.89 (0.84-0.94) 0.83 (0.76-0.89) 0.92 (0.88-0.96) 0.74 (0.66-0.82) 0.58 (0.49-0.67) 0.78 (0.71-0.85) 0.94 (0.90-0.98) Sens (%) 83 68 82 89 71 89 50 28 56 89 Spec (%) 78 98 92 80 84 82 91 95 95 89 AUC (LOOCV) 0.85 0.87 0.89 0.89 0.82 0.92 0.73 0.55 0.76 0.92 Sens (%) 78 68 82 85 68 88 47 28 56 86 Spec (%) 82 98 90 82 86 83 91 90 94 90 Self-sample ADCYAP1 BHLHE22 CDH13 CDO1 GALR1 GHSR HAND2 SST ZIC1 CDO1+GHSR+ZIC1 AUC (non-CV; 95%-CI) 0.69 (0.60-0.78) 0.78 (0.70-0.85) 0.70 (0.62-0.79) 0.90 (0.84-0.95) 0.74 (0.66-0.82) 0.83 (0.77-0.90) 0.69 (0.58-0.75) 0.61 (0.52-0.69) 0.62 (0.53-0.70) 0.92 (0.87-0.97) Sens (%) 53 64 49 75 51 71 50 35 54 86 Spec (%) 93 89 93 94 95 87 88 89 68 92 AUC (LOOCV) 0.68 0.77 0.69 0.89 0.73 0.83 0.65 0.58 0.57 0.91 Sens (%) 53 62 47 78 51 65 47 33 54 83 Spec (%) 92 90 93 91 93 91 88 87 63 92 Scrape ADCYAP1 BHLHE22 CDH13 CDO1 GALR1 GHSR HAND2 SST ZIC1 CDH13+CDO1+ZIC1 AUC (non-CV; 95%-CI) 0.82 (0.76-0.89) 0.86 (0.80-0.92) 0.69 (0.60-0.77) 0.95 (0.92-0.98) 0.62 (0.53-0.71) 0.89 (0.84-0.94) 0.60 (0.51-0.70) 0.70 (0.62-0.78) 0.76 (0.68-0.83) 0.97 (0.95-0.99) Sens (%) 71 75 46 82 53 75 40 60 78 93 Spec (%) 80 85 90 94 76 93 86 75 67 89 AUC (LOOCV) 0.82 0.85 0.67 0.95 0.59 0.88 0.57 0.69 0.74 0.96 Sens (%) 60 74 46 89 53 76 39 44 78 89 Spec (%) 91 85 90 86 75 90 84 89 65 90 Individual methylation markers and marker panels with the highest AUC value per sample type are depicted in bold. AUCs, including 95% Cl, are reported together with sensitivities and specificities based on the maximal Youden’s Index (J) threshold. AUC = area under the receiver operating characteristic curve; LOOCV = leaveone-out cross-validated; Sens = sensitivity; Spec = specificity. 4
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