161 Diversity of the gut microbiota in original and IgG-coated fecal samples Microbial richness and diversity of both untreated (full fecal microbiome) and IgG-coated fecal samples are presented in Figure 1. The median number of operational taxonomic units (OTUs) per sample, representing sample richness of the gut microbiota, was significantly lower in samples of patients with IBD compared with samples from HC (298 [interquartile range [IQR]: 200-467] vs. 625 [IQR: 504-717], P<0.001) (Figure 1A). No significant paired difference in OTU counts was observed between original and IgG-coated fecal samples of HC (635 [IQR:512-724] vs. 624 [IQR: 497-696], P>0.05), whereas IgG-coated fecal samples from patients with IBD showed a decreased OTU count (340 [206-530] vs. 287 [185-436], P<0.001) compared with untreated fecal samples. The median Shannon diversity index, which represents the alpha-diversity (richness and diversity) of the gut microbiota, was significantly lower in IBD samples compared with samples from HC (3.2 [IQR: 2.7-3.5] vs. 4.1 [IQR: 3.8-4.3], P<0.001) (Figure 1B). Similar to the OTU counts, no paired difference was observed between untreated and IgG-coated fecal samples from HC (4.2 [IQR: 3.8-4.4] vs. 4.0 [3.8-4.3], P>0.05), whereas there was a small difference in untreated vs. IgG-coated samples from patients with IBD (3.2 [IQR: 2.9-3.7] vs. 3.1 [2.7-3.4], P<0.001). Antimicrobial IgG immune responses in IBD
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