205 Tertiary objectives 1. A decrease in salivary flow could influence oral hygiene. Oral hygiene and bacterial composite can be analyzed by 16S rRNA sequencing of saliva.93 The tertiary aim of the study is to determine the bacterial composition in saliva samples using 16S rRNA Illumina sequencing and monitor potential changes in disease-associated species. The relative abundance of bacteria in saliva at baseline (before surgery) will be compared to samples 8 and 32 weeks after surgery. At baseline, the 8, and the 32 weeks measurements, we will take a 1 mL sample of unstimulated saliva for subsequent saliva sequencing. To limit the effect of dietary components on the bacterial composition, we will ask patients to only drink water during the 1.5 hours before the measurements. Samples are stored anonymously at -80 degrees Celsius. The DNA will be isolated from the saliva as described by Rosier et al.93 The bacterial composition will then be determined using 16S rRNA Illumina sequencing and the DADA2 pipeline for taxonomic classification as defined by Johnston et al. Samples will be compared with different statistical methods as described previously.93, 94 If there is insufficient saliva to collect 1 mL saliva, we will use cotton pledges to collect saliva. The cotton pledges will then be squeezed to collect liquid saliva. Study design A prospective study in children and adolescents who would undergo submandibular salivary gland surgery to treat drooling and are willing to participate. Study population All patients who opt and qualify for submandibular gland surgery to treat anterior drooling.
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